Narishige Pc10 Manual New - ((better))

The Narishige PC-10 is a dual-stage, vertical micropipette puller that has become a standard in laboratories for decades. Its principle is beautifully simple, yet its execution is highly precise. The instrument uses a vertical heating element to melt a glass capillary tube. As the glass softens, the puller relies on the gravitational force of its own weight, supplemented by adjustable weights, to pull the capillary into two separate, fine-tipped micropipettes. This method allows for the consistent fabrication of pipettes with tips ranging from one micron down to as small as one-tenth of a micron.

Gently guide the bottom end of the glass into the lower clamp, then tighten the lower thumbscrew. Do not overtighten, as this can crack the glass before the pull begins. Step 3: Setting the Variables

Loosen the two thumb screws or terminal blocks holding the coil in place. narishige pc10 manual new

Turn off power. Inspect the coil for breaks. Tighten holding screws. Overheating or uneven cooling air.

Position the PC-10 on a stable, vibration-free laboratory bench. Keep the surrounding area clear of flammable materials, solvents, or volatile chemicals. The Narishige PC-10 is a dual-stage, vertical micropipette

Detailed operational settings and maintenance tips can be found in the Narishige PC-10 User Manual.

The PC-10 is (e.g., steep taper for nucleated patches) but works fine for standard whole-cell and sharp electrodes. As the glass softens, the puller relies on

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